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KMID : 1234920130130020074
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Journal of Korean Oriental Association for Study of Obesity
2013 Volume.13 No. 2 p.74 ~ p.83
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Effects of Fermented Lotus Extracts on the Differentiation in 3T3-L1 Preadipocytes
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Lee Sin-Ji
Shambhunath Bose
Lee Su-Jin
Jung Ji-Eun
Koo Byung-Su
Kim Dong-Il
Kim Ho-Jun
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Abstract
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Objectives: This study was performed to evaluate the effects of fermented lotus extracts on the inhibition of differentiation in 3T3-L1 preadipocytes.
Methods: Extracts of lotus leaf and lotus root were fermented using 4 different probiotics separately, including Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium breve, and Bifidobacterium longum. Inhibition of preadipocyte differentiation was examined by Oil red O dye staining. Expressions of adipogenic transcription factors including CCAAT/enhancer binding proteins (C/EBPα) and peroxisome proliferators-activated receptor γ (PPARγ) were analyzed by real time polymerase chain reaction and Western blotting analysis.
Results: Fermented lotus extracts inhibited adipogenic transcription factors by inhibiting preadipocytes differentiation. All of the groups fermented by 4 kinds of probiotics showed reduction in Oil Red O dye staining. Bifidobacterium breve showed the most effective inhibition of C/EBPα. Bifidobacterium breve and Bifidobacterium longum showed the best downregulation of PPARγ expressions compared with the control and the unfermented lotus group.
Conclusions: Fermented lotus extracts showed significant effects on inhibition of preadipocyte differentiation in 3T3-L1 preadipocytes showing correlation with insulin sensitivity and lipid metabolism related with obesity.
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KEYWORD
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3T3-L1 preadipocyte, Lotus, Probiotics, Fermentation, Obesity, Insulin resistan
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